1Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2Chronic Kidney Disease Research Center, Department of Nephrology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Department of immunology, School of medicine, Tehran University of Medical Sciences, Tehran, Iran and Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Background: The innate immunity plays an important role in the host response to transplantation by Toll-like receptors and results in development of acute allograft rejection. The aim of this study was to evaluate the association of TLR2 and CD14 (co-receptor) gene polymorphisms with acute rejection in kidney transplant recipients.
Methods : The study was conducted in a population of 239 subjects consisting of 71 patients with acute rejection, 71 patients without acute rejection (SGF) and 97 Healthy Control (HC). The allele and genotype frequencies of TLR2 (R753Q, rs5743708) and CD14 (-159 C>T, rs2569190) polymorphisms were genotyped by Real-time PCR in the study groups.
Results : Genotype distribution of CD14 -159 polymorphism was significantly different in AR vs. SGF and HC. CD14 -159 TT genotype was more prevalent in rejection than SGF and HC (P<0.0001, P<0.007, respectively). Also Graft loss, defiened as need of dialysis after acute rejection, was occurred in 24 patients (33.8%) from AR group. The frequencies of three genotype in CD14 (TT, CT, CC) in rejection With Graft loss were 75.0%, 20.8% and 4.1% respectively, While 25.5%, 31.9% and 42.5% in rejection without Graft loss (P<0.0001 for TT vs. CT, CC). Many recipients with AR were involved with graft loss had CD14 -159 TT genotype, whereas only a few recipients without graft loss had TT genotype (p<0.0001).
Conclusion : Therefore, due to the importance of CD14 polymorphism (-159 C/T, rs2569190) in disease progression and also as a biomarker, could be considered as a crucial therapeutic target in early prognosis of acute rejection