Molecular and Biochemical Diagnosis Journal
Molecular and Biochemical Diagnosis Journal
Molecular and Biochemical Diagnosis Journal
Medical Sciences
http://mbd.modares.ac.ir
1
admin
2383-0522
2476-6607
2267
en
jalali
1393
1
1
gregorian
2014
4
1
1
1
online
1
fulltext
en
Development of a SYBR green real time multiplex RT-PCR technique for simultaneous detection of HCV and GBV-C Co-infection in plasma samples
Background:Accumulative research is in progress to clarify clinical aspects of GBV-C. The possibility of interaction between HCV and GBV-C as well as its consequence on development of liver diseases is the most important clinical aspect which encourages researchers to develop a rapid and cost effective technique for simultaneous detection of both viruses. Methods: In this study, a SYBR Green real time multiplex RT-PCR technique as a new economical and sensitive method was designed and validated for simultaneous detection of HCV/GBV-C in HCV positive plasma samples. SYBR green real time RT-PCR technique optimization was performed separately for each virus. Multiplex PCR was established next. Standard sera with known concentrations of HCV RNA and dual HCV/GBV-C positive control samples along with negative control samples were used to validate the assay. Results and Conclusions: Fifty six non cirrhotic HCV positive plasma samples [29 of genotype 3a and 27 of genotype 1a] were collected from patients before receiving treatment. 20.6% of genotype 3a and 18.7% of genotype 1a showed HCV/GBV-C co-infection. As a result, 19.6% of 56 samples had HCV/GBV-C co-infection that was compatible with other results from all over the world. SYBR Green real time multiplex RT-PCR technique can be used to detect HCV/GBV-C co-infection in plasma samples. Furthermore, with application of this method more time and cost could be saved in clinical-research settings.
HCV,GBV-C,SYBR Green real time,Rapid detection,multiplex RT- PCR
41
49
http://mbd.modares.ac.ir/browse.php?a_code=A-10-1000-2197&slc_lang=en&sid=8
Seyed Hossein
Mousavi Fard
Seyed Hossein
Mousavi Fard
100319475328460053834
100319475328460053834
No
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Shahin
Merat
Shahin
Merat
100319475328460053833
100319475328460053833
No
Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran
Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran
Kiana
Shahzamani
Kiana
Shahzamani
100319475328460053832
100319475328460053832
No
Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Reza
Ghanbari
Reza
Ghanbari
100319475328460053831
100319475328460053831
No
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
Neda
Yahoo
Neda
Yahoo
100319475328460053830
100319475328460053830
No
Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Farzaneh
Sabahi
Farzaneh
Sabahi
100319475328460053829
100319475328460053829
Yes
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran