Molecular and Biochemical Diagnosis Journal
Molecular and Biochemical Diagnosis Journal
Molecular and Biochemical Diagnosis Journal
Medical Sciences
http://mbd.modares.ac.ir
1
admin
2383-0522
2476-6607
2267
en
jalali
1393
7
1
gregorian
2014
10
1
1
3
online
1
fulltext
en
Identification of the Propionibacterium acnes using Polymerase Chain Reaction (PCR) in the Acne vulgaris lesions
Background: Acne vulgaris is an inflammatory chronic disease of pilosebaceous unit. One of the most important factors playing a role in occurrence of acne is presence of <em>Propionibacterium acnes</em>. With the aim of molecular identification of the P. acnes from the acne vulgaris lesions, current research was carried out. Methods: In this study, contents within the lesions was collected from 70 patients. The presence of the <em>P. acnes</em> was examined by a specific PCR technique. Results: Of 70 samples, 58 samples (82.85%) were determined to be positive in terms of presence of <em>P. acnes</em>. No significant relationship was observed between presence of <em>P. acnes</em> and each one of the studied demographic factors, including gender, age, disease period, family background and treatment background. Conclusions: The adopted molecular technique has obviated the limitations associated with the culture method for identification of the bacteria. To overcome the problems with conventional culture techniques for <em>P. acne</em>, this PCR method is promising for better identification of this bacterium.
identification,Polymerase Chain Reaction (PCR),Propionibacterium acnes,Acne vulgaris
205
212
http://mbd.modares.ac.ir/browse.php?a_code=A-10-1000-6347&slc_lang=en&sid=8
Masood
Ghane
Masood
Ghane
100319475328460060645
100319475328460060645
No
Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
Negin
Naghdi
Negin
Naghdi
100319475328460060646
100319475328460060646
No
Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran