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2014, 1(1): 41-49 |
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Mousavi Fard S H, Merat S, Shahzamani K, Ghanbari R, Yahoo N, Sabahi F. Development of a SYBR green real time multiplex RT-PCR technique for simultaneous detection of HCV and GBV-C Co-infection in plasma samples. Molecular and Biochemical Diagnosis Journal 2014; 1 (1) :41-49
URL: http://mbd.modares.ac.ir/article-8-2898-en.html
URL: http://mbd.modares.ac.ir/article-8-2898-en.html
Seyed Hossein Mousavi Fard1 
, Shahin Merat2 , Kiana Shahzamani3 , Reza Ghanbari4 , Neda Yahoo3 , Farzaneh Sabahi 1
, Shahin Merat2 , Kiana Shahzamani3 , Reza Ghanbari4 , Neda Yahoo3 , Farzaneh Sabahi 1
1- Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
2- Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
4- Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
2- Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
4- Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
Abstract: (9535 Views)
Background:Accumulative research is in progress to clarify clinical aspects of GBV-C. The possibility of interaction between HCV and GBV-C as well as its consequence on development of liver diseases is the most important clinical aspect which encourages researchers to develop a rapid and cost effective technique for simultaneous detection of both viruses. Methods: In this study, a SYBR Green real time multiplex RT-PCR technique as a new economical and sensitive method was designed and validated for simultaneous detection of HCV/GBV-C in HCV positive plasma samples. SYBR green real time RT-PCR technique optimization was performed separately for each virus. Multiplex PCR was established next. Standard sera with known concentrations of HCV RNA and dual HCV/GBV-C positive control samples along with negative control samples were used to validate the assay. Results and Conclusions: Fifty six non cirrhotic HCV positive plasma samples [29 of genotype 3a and 27 of genotype 1a] were collected from patients before receiving treatment. 20.6% of genotype 3a and 18.7% of genotype 1a showed HCV/GBV-C co-infection. As a result, 19.6% of 56 samples had HCV/GBV-C co-infection that was compatible with other results from all over the world. SYBR Green real time multiplex RT-PCR technique can be used to detect HCV/GBV-C co-infection in plasma samples. Furthermore, with application of this method more time and cost could be saved in clinical-research settings.
Received: 2013/10/14 | Accepted: 2013/12/14 | Published: 2014/01/1
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